Licefreee Spray kills nits as well as lice so the nits left in your hair after treatment would be dead as long as they came in contact with the product and it was allowed to dry naturally. Dead nits will not fall off the hair follicle, they will stay attached and grow out with your hair. Some schools do not have a no-nit policy and do not require removal of the nits, so in that instance removing the nits is your choice.