Although Licefreee Spray is non-toxic and safe to use as often as you like, it is not necessary to apply the treatment more than one time in a day. It is better to continue to use the nit comb daily to remove any nits you may see in the hair since it may be difficult to find them all in one session. We recommend using Licefreee within 7-10 days  to make sure all lice have been killed, or if you see new lice in the hair.