Yes, Licefreee Gel does kill the eggs as well as long as the product is fully in contact with the egg for a full hour. However, it is best practice to remove all of the eggs using the included nit comb just in case the product did not fully come in contact with that egg. Also note you can use the nit comb without doing a full treatment over the next few days just in case you missed any nits during the comb through. They can be tough to find.